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Each plant should be carefully removed from its tube of medium and planted into a small pot containing a clean light potting mix. Gently wash off all of the agar medium prior to planting. The plants will still need to be protected at this stage since they are not accclimated to the drier air of the classroom when compared to the moist environment of the tube. Place all of the pots onto a tray and cover loosely with a plastic dome or tent. Place the plants in an area with 12-16 hours of light (either natural or artificial) but not direct sunlight.

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The most important part of this activity, however, is to maintain as sterile an environment as possible. Even one fungal spore or bacterial cell that comes into contact with the growth medium will rapidly reproduce and soon completely overwhelm the small plant piece that you are trying to clone. If you wish to use plants other than cauliflower, you need to prepare two different media which contain plant hormones necessary to stimulate development of differentiated tissues. The first one should contain a cytokinin such as BAP which promotes shoot formation and the second one a rooting hormone such as NAA or store bought rooting hormone. To do this, prepare the mixture up until the end of step 2. Keeping the mixture warm so that it does not solidify, divide it equally into two pre-warmed containers.

However, high sucrose concentration in the media restricts the photosynthetic efficiency of cultured plants by reducing the key enzymes for photosynthesis, levels of chlorophyll, and epicuticular waxes promoting the formation of structurally and physiologically abnormal stomata. The most preferred carbon or energy source is sucrose at a concentration of 20–60 g/L. But the levels of sucrose that are normally used to support the growth of tissue cultures are often inhibitory to chlorophyll synthesis.

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